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Both human [87] and mouse studies [88, 89] demonstrated that a Plin1 deficiency enhanced ectopic fat distribution and impaired insulin sensitivity

The extent of the downregulation depends on interactions between Sp1 and PPAR [117]

This emphasizes the critical need for proper aseptic handling and storage to maintain sterility over repeated use

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If the vial was out for 8+ hours at room temperature (2025C), the conservative approach is to discard it and start a fresh vial