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Then, the cells were resuspended in 0.2 M phosphate buffer (pH7.0) containing different concentrations of l-cysteine or GSH and 0.5 % (v/v) toluene, and the reaction mixture was incubated at 37 C for 2 h

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2.9 Glutathione content measurement Tissue samples (1.5 g) were ground in 2 mL 60 mmol L 1 sodium phosphate buffer (pH 7.0), and refrigerated centrifugation was performed at 12,000 g for 15 min, after which 100 L of supernatant was obtained

advantus (imidacloprid) Soft Chew Indications: Use: advantus kills adult fleas and is indicated for the treatment of flea infestations on dogs and puppies 10 weeks of age and older and weighing 4 pounds or greater