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Data was further normalized by dividing each sample by its sum of UMIs, resulting in normalized expression levels corresponding to the genes fraction in the sample

In our opinion, the increase in hydrogen sulfide production in glutathione-treated old animals was due to an increase in the expression of 3-MST (by 25%), or probably the activity of this mitochondrial enzyme due to an excess of the substrate L-cysteine, which is common for the synthesis of this gaseous mediator and glutathione

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