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The tissue was then digested for 15 min in a 37 C shaking water bath incubator using 5 ml of 100 U/ml collagenase type I (Sigma) in Basal Medium Eagle (Thermo Fisher Scientific) and supplemented with 1% BSA, 26.4 mM N a HCO 3 , and 10 mM HEPES (pH 6.9)

J., Santos-Parker, J

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Before mixing (lyophilized / freeze dried Epitalon powder): refrigerated between 2 - 8 degrees celsius (36 - 46 Fahrenheit) it can be used for 24 months at room temperature (up to 37 degrees Celsius or 98.6 Fahrenheit) it can be used for over 90 days After reconstitution (liquid Epithalon): liquid Epithalamin should be refrigerated between 2 - 8 degrees celsius (36 - 46 Fahrenheit) at all times