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7.2.1 Prodrug Design SAG adopts advanced prodrug technology, a mature approach widely used in the pharmaceutical industry
The following day medium was removed and replaced with 20 l of loading medium consisting of 1:1 (v/v) Opti-MEM: Hanks balanced salt solution (HBSS), 2.5% (v/v) FBS, 20 mM HEPES, pH 7.4, 2.5 mM probenicid and fluorescent indicator 2 M Fluo-4 AM (Invitrogen)
Owing to the variety of contributing factors and prolonged nature of UC, the development of new clinical drugs and novel personalized treatments are research priorities
The PZQ LD 50 for NTS was determined after just 24 h exposure using both the Cell TiterGlo (27.1 2.27 M, Table 2) and the phenotypic analysis (42.9 0.83 M, Supplementary Movie 2), which is also used by others 54
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