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From a 5 mg vial reconstituted with 2 mL (2.5 mg/mL), 0.25 mg is 0.10 mL, which reads as 10 units on a U-100 insulin syringe

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Importantly, the MHRA has issued a Drug Safety Update warning that biotin (vitamin B7) supplementation can interfere with a range of laboratory immunoassays, potentially causing false results