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Additionally, cAMP and PKA phosphorylate ERK1/2, which controls genes involved in proliferative and anti-apoptotic processes, and activate several proteins, such as the mitogen activated protein kinase (MAPK) cascades [63]

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Media was exchanged after the overnight incubation, and then into selection media containing 2 ug/mL puromycin (Sigma-Aldrich, P9620) after 24 hours