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Olympus, Australia), and image quantification of thickness (in micrometers), live and dead biovolumes (square micrometers per cubic micrometers), and substratum surface coverage (in square micrometers), using ImageJ (v1.49), were performed as described previously (21)

Viruses 7 , 945953 (2013)

Quantitative real-time PCR Total RNA was isolated from tissue or cells using Qiazol and RNeasy mini kit (Qiagen, Venlo, Netherlands) according to manufacturers instructions and with 15 min on-column DNase digestion (Qiagen, Venlo, Netherlands)

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There are three forms of mercury in the environment: elemental, found in batteries, thermometers, and dental amalgams