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Structure-Bioactivity Relationships of Methylxanthines: Trying to Make Sense of All the Promises and the Drawbacks

Fully oxidized and reduced states of roGFP2 with maximum and minimum fluorescence ratios at 390/480 nm were determined with 10 mM H 2 O 2 and 10 mM DTT, respectively

To assess if tirzepatide and SAR441255 compete with [ 68 Ga]Ga-DO3A-Exendin-4 for binding to GLP-1R, frozen huGLP-1R-HEK293 cell pellet sections were pre- incubated with either 10 M tirzepatide, 10 M SAR441255 (both compounds were generated by custom in-house Solid Phase Peptide Synthesis (SPPS) by Sano), or 1 M DO3A-Exendin-4 (positive control)