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Subsequent experiments showed that the active forms of GLP-1 were GLP-1(736 amide) and GLP-1(737) isoforms, increasing cAMP levels, insulin mRNA transcripts, promoting insulin secretion in the pancreases of pigs and rats, respectively [19]

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To validate these findings, we assessed glucose uptake rates through flow cytometric measurement of a fluorescently labelled glucose analogue (2-NBDG)

Within-cell analyses in human-GLP1R-expressing neurons confirmed depolarization from baseline and a reduced time to spike threshold during current injection, indicating increased excitability following danuglipron application (Extended Data Fig