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Far from being a bystander, mediation analysis indicated that TMAO significantly accelerated the rate of kidney function decline by age, with its effect increasing in more severe disease stages

95% CI, 0.09 to 0.15

To execute this, 200 L of the examined samples, previously diluted with PBS at a 1:5 ratio (v/v), were combined with standard solutions ranging from 0 to 100 mol/L alongside 200 L of blank PBS solution

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