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-lipoic acid inhibits sevoflurane-induced neuronal apoptosis through PI3K/Akt signalling pathway

Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron- dependent accumulation of lipid reactive oxygen species (PubMed:24439385)

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If required, cells were transfected with plasmid DNA using jetPRIME according to the recommended protocol of the supplier, one to two days before imaging