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Many factors can affect the efficiency of cell-free gene expression including vector sequence, reaction components and the template DNA concentration

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Immune effects include increased IL-2, reduced IL-6, and enhanced NK cell activity, while DHEA/DHEA-S deficiency combined with excess cortisol contributes to immune dysregulation

Subcutaneous injection allows gradual systemic absorption through adipose tissue

Future Phase III trials (e.g., ongoing photoaging studies) may further clarify its utility [12]

Hepatic: q-value: 1.2 * 10 7 , 83 DEGs out of 283 genes, 54.8% downregulated), which were post-transcriptional protein modifications necessary for transport of proteins from the ER to the Golgi 17