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biliary copper excretion), and GHK-Cus capacity to modulate metallothionein expression, collagen remodelling, antioxidant gene programmes, and hepatic stellate cell (HSC) biology positions it as a mechanistically important tool in liver fibrosis and hepatoprotection research

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The cells were further incubated for 6 h and 50 L of luciferase substrate (InvivoGen) was applied to read the luminescent signal on Synergy 4 Microplate Reader (BioTek)

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