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For the optimization of culture conditions, medium component screening experiments were performed in which each of the components Amphiregulin (AREG

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The PCR positive clones were further confirmed by restriction digestion analysis of recombinant His6-AV-Ubiquitin-Semapeptide (nucleotide sequence)- pET24 plasmid vectors using Ndel (NEB) + Xhol (NEB) at 37 C

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