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Methods Tissue preparations All animal procedures were approved by the University of Cambridge Animal Welfare and Ethical Review Body and were conducted in accordance with the UK Animals (Scientific Procedures) Act 1986 Amendment Regulations (SI 2012/3039)

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Short chain acylcarnitines have attracted a lot of attention in the context of diabetes and insulin resistance, with acetylcarnitine playing again a central role as a master regulator of energy metabolism strategies

However, a larger increase in glutamate content without concomitant decrease in aspartate observed in an epileptic patient almost certainly represents increased de novo synthesis (Mangia et al., 2012)

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