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This flexibility represents an important practical advantage, allowing patients to choose a schedule that best fits their lifestyle and daily routine

Immunocytochemistry Cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) for 10 min at 20 C, washed three times with PBS, and then incubated with blocking buffer (1% FBS and 1% BSA in PBS) for 1 h at 20 C

It seems you may need high B12 supplementation and to stop any folate supplements for a while

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