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Avoid vigorous shaking or excessive agitation when reconstituting or handling the vial

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Aging, diabetes, obesity, liver disease, chronic infections, pollution exposure, alcohol intake, and long-term medication use accelerate this depletion

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Controls simultaneously received an equivolume of saline (5.0 ml/kg ip) or water only (12 ml/rat/day)

Mudgal and coworkers demonstrated that in Swiss mice treated with LPS, a one-week treatment with a low dose of caffeine and a low dose of caffeic acid produced anxiolytic effects in the OFT and DLB, antidepressant effects in OFT and DLB, and anti-inflammatory effects with reduction of IL-6 and TNF-alpha in serum and brain [34]