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After 10 min incubation at 4 C, samples were centrifuged at 20,000 rpm for 5 min, and 600 L of supernatant was transferred to the new 2-mL tubes and completely dried in a vacuum concentrator for 4 h

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THC is capable of reducing the minimum effective dose of morphine by 55%, methadone by 75% and codeine by 96% [52]