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U2OS cells were cultured in McCoys 5A with 10% FCS (Gibco, Invitrogen) and 1% glutamax, 0.5 g/ml hydrocortisone

This is where the debate truly begins

BPC-157s exact half-life in humans has not been formally established through clinical pharmacokinetic studies

As a result, it has become a key area of research in aging, regenerative medicine, and treatments for age-related diseases

A third widely-cited paper (McCoy 2013, PMID 23055539) the source of the often-repeated "seven orders of magnitude more potent than BDNF" line carries a 2021 Expression of Concern

The qPCR cycling conditions were as follows: an initial denaturation at 95 C for 15 min, followed by 30 cycles of 95 C for 7 s and 58 C for 10 s, and a final extension step at 95 C for 5 min, then 40 cycles of 95 C for 15 s and 58 C for 30 s