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After drug treatment for indicated time, 500 nM final concentration of SYTOX Green Nucleic Acid dye was added into each well, cells were further incubated at 37 C for 15 min, and images of at least three randomly chosen fields were captured by an inverted fluorescence microscope (Olympus, Japan)

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Research has investigated whether Epithalon influences hTERT expression, potentially leading to increased telomerase activity in cells that normally show limited enzyme function

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