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For a 1 mg (1,000 mcg) target dose: Volume to Draw = Desired DoseConcentration = 1 mg5 mg/mL = 0.2 mL Convert to syringe units

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Following incubation, plates were washed three times with 25X DELFIA Wash Concentrate (Perkin Elmer Cat #1244-114) diluted to 1X and incubated for one hour with an internal antibody against M13 pre-conjugated to DELFIA Eu-N1 Rabbit Anti-Mouse-IgG antibody (Perkin Elmer Cat #AD0207) diluted in DELFIA Assay Buffer (Perkin Elmer Cat #4002-0010)

It may be necessary to undergo multiple treatments in order to achieve optimal results

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These effects, when present, are typically manageable and resolve with continued use or dose adjustment