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The culture medium was removed, and the cells were incubated with serum-free DMEM containing 10 M DCFH-DA at 37 C for 20 min

Compounding pharmacies are a small percentage of the larger pharmacy industry only around one percent of people use them, according to one study but they serve an important role in medication supply and can offer a lot of benefits to certain patients

Samples were resuspended in 160 L EPPS buffer (200 mM, pH 8.0) using a probe sonicator (1 1015 pulses, power output 20%) and then digested with LysC (4 L of 0.5 g/L per sample, resuspended in HPLC grade water, Wako-chemicals, Fujifilm #125-05061) for 2 h at 37 C in a shaker incubator

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The source data generated in this study are provided in the Supplementary Information/Source Data file

therefore, they have become a potential management issue perioperatively [37]