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Membranes were sequentially incubated with primary antibodies (anti-GLP-1 [Santa Cruz, sc-80603, 1:1,000], anti-MPC2 [Proteintech, 20049-1-AP, 1:2000], anti-GAPDH [Cell Signaling, D4C6R-97166, 1:5,000]) overnight at 4 C, followed by HRP-conjugated secondary antibodies (Santa Cruz, sc-2354, 1:5,000) for 1 h at RT

Patients should aim for 6-8 glasses of fluid daily (about 1.5-2 litres), adjusting for individual needs, hot weather, or physical activity

Staying hydrated: What works best and how much is needed Aim for a consistent daily intake to support regular bowel movements and overall health

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Willow did not respond to requests seeking comment about its marketing

Nee, R., Yuan, C