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14 Resulting cell suspensions were centrifuged (5 min, 300 g ) and the pellets were resuspended in DMEM (supplemented with 10% fetal calf serum and 1% antibiotics, 100 U ml 1 penicillin and 0.1 mg ml 1 streptomycin)

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To evaluate the short-term temporal dynamics of methylation and demethylation effects, we measured the expressions of epigenetic modifying enzyme genes

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