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It is characterized by cell expansion, plasma membrane pores creation, rupture, and proinflammatory mediators leakage, including interleukin 1 beta (IL-1) and IL-18 into the outside of cells, amplifying the local or systemic inflammation [12]
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Junction PCR confirmed the knock-in event in the liver, and digital PCR indicated an efficiency of approximately 1% for a single administration of Donor+Cas9, consistent with previously reported in vivo knock-in efficiencies (Fig
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fumigatus strains ATCC46645 and ATCC26933 were co-transformed with two DNA constructs, each containing an incomplete fragment of a pyrithiamine resistance gene ( ptrA ) [21] fused to 1.2 kb, and 1.3 kb of gliT flanking sequences, respectively
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