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Additional elements of the expression cassette included an FMDV IRES upstream of P1, the FMDV 3UTR downstream of 3CD followed by a 20 nucleotide long polyA tail, as well as the T7 promoter and terminator elements 15

Preliminary studies suggest that adherence to the MD is linked to reduced oxidative damage in spermatozoa and improved semen quality, possibly due to enhanced mitochondrial efficiency and redox balance [4]

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P 1.5 were considered significantly differentially expressed proteins 7

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Muscle protein is in a constant state of turnover, meaning that protein synthesis is occurring continuously to replace protein lost as a consequence of protein breakdown