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117 The film-forming and barrier effects of this medical device were studied using a 3D reconstructed human esophageal epithelium model
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The fitted models were further refined by rounds of manual model building in COOT 54 and real-space refinement, as implemented in the PHENIX software package 55
Some cells were labeled with the F-actin specific probe Phalloidin (diluted 1:200 in PBS) for 40 min at 37 C
137,138 Structural models of DPP4 and its inhibitors, as well as the viral spike proteins of MERS-CoV and SARS-CoV-2, indicate that the binding sites for DPP4 inhibitors do not overlap with those for viral spike proteins
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