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The final purified samples were desalted into phosphate buffer saline (PBS) for later tests and separated on a gel with a concentration of 12.5% SDS-PAGE

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The peptide is supplied as a blue-tinged lyophilized powder at 50mg per vial, with purity exceeding 98% by HPLC analysis, and includes verified copper content by atomic absorption spectroscopy

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The stained samples were analyzed using a BX53 microscope and a confocal microscope (BX61 type FV1000D, Olympus Corporation)

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