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The method provided by Lowry et al

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DOI: 10.1016/j.jneuroim.2007.01.008 35

GLP1Rs are G-protein-coupled receptors (GPCRs) and previous studies largely in peripheral tissues report that GLP1R agonism increases levels of the secondary messenger cyclic adenosine monophosphate (cAMP) through the stimulatory alpha subunit G protein (Gs) protein signaling pathway to mediate its effects 1822