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Tissues were homogenized in RIPA buffer (Tris (50 mM), NaCl (150 mM), Triton X-100 (1%), disodium EDTA (1 mM), SDS (0.1%), sodium deoxycholate (0.5%)) containing protease and phosphatase inhibitors (S8830, SigmaFast, Thermo Scientific) followed with mild sonication (5 min, cycle: 30 s on/off

Immunoglobulin-A levels and expression of antimicrobial peptides are gastrointestinal markers that provide insight into gut immunity and potential etiology of gut-centric disease processes 31

2003 Dec;29(6):501513

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