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Beyond NSA-involving metals in the LC flow path, we also believe that ionic interactions between positively charged sites on peptides or related impurities and residual siloxide groups on commonly used silica particles can exacerbate NSA, leading to sensitivity issues and poor peak shapes

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This longer gap increases the risk of appetite return and weight regain, so most providers avoid it unless specifically indicated for side effect management

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Available from: Devarapalli S, Blaske B, Henson, Henson J, et al