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For the SPRDtgHD rat tissues, 8-month-old animals were killed via transcardial perfusion with perfusion buffer (0.01 M PBS, pH 7.4)

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They show some positive effects on sleep quality, such as restful sleep, but they dont show that melatonin supplements reverse the cognitive deficits of AD.[ref] One placebo-controlled trial of six months of 2mg time-release melatonin in people with mild to moderate AD did show positive results on cognitive performance compared to placebo