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Live LMMP preparations were incubated with 4 M calcein AM for 30 min at 37C and then immediately imaged using a Neo scientific complementary metal-oxide semiconductor (sCMOS) digital camera (Andor, South Windsor, CT) through the 40 water immersion objective (LUMPlan N, 0.8 numerical aperture) of an upright Olympus BX51W1 fixed-stage microscope controlled by Andor IQ3 software

Instead, the FDA removed enforcement priority for these compounds, effectively treating them as neither affirmed nor prohibited in the bulk substance framework

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