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Protein pellets were extracted from these aliquots by two rounds of centrifugation at 16,000 rpm x g, first for 10 minutes, and then for 5 minutes after washing with 50 L of chilled acetone

The composition distances of gut microbiota between the NC and PCOS groups were dispersed, while the composition distances of samples in the same group were relatively concentrated, suggesting that the structure of the bacterial community was different between the two groups

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