Cells were gently resuspended in tubes containing 10 mL of pre-warmed RPMI 1640 media (Gibco) supplemented with 10% FBS (Biological Industries), L-glutamine (2 mM), penicillin (100 U/mL, Biological Industries), streptomycin (0.1 mg/mL, Biological Industries) and Benzonase Nuclease (1:10,000, Millipore), and centrifuged for 7 min at 400 g , RT
p = 0.002), triglycerides (TG) (ES = 2.51 mg/dL
Step 2: Hydrate & Reflect
Serena was transparent about why she chose this method
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This will help to ensure that you are getting enough vitamin B12