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although in some cases, the macules may be confused for melanoma in situ (a precancerous skin condition) In a majority, no treatment of Vulvar Melanotic Macules may be necessary and the healthcare provider may undertake a wait and watch approach

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Can J Biochem

Z.et al (2023)

Total Cholesterol (TC) 0.5ml of working reagent was added to a set of clean labeled test tubes and two of the test tubes were labeled standard 1 and 2 while another was labeled blank.0.1ml of homogenate was added to the approximately labeled test tube .mixed and incubated for 30 minutes at 37, the absorbance of the sample and standard were read against the reagent blank at the wavelength of 546nm