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Add 20 L of the diluted GR Positive Control, Assay Buffer (used as a blank) or unknown samples to a 96-well microtiter plate

Diana Aguiar de Sousa: No disclosures related with the specific topic, but has received personal fees from AstraZeneca, Organon, and Johnson & Johnson for advisory board participation, DSMB participation for the SECRET trial (University of British Columbia), and speaking fees from Astrazeneca and Bial

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NADPH oxidase-mediated reactive oxygen species production activates hypoxia-inducible factor-1 (HIF-1) via the ERK pathway after hyperthermia treatment