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A key insight was the realization that the nausea seen after injections of liraglutide (Victoza) could be mitigated if one started at a lower dose and then gradually increased it

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RP-HPLC purification was conducted by collection under the same conditions on a semi-prep scale using a Grace Vydac Denali C18, 120 , 150 10.00 mm, and 5 m column at a 5 mL/min flow rate