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The oocytes were subsequently incubated in a permeabilizing solution (0.5% Triton-X-100 in PBS-PVA) for 30 min at room temperature, washed twice in PBS-PVA, and then blocked with 2% FCS in PBS for 45 min

Ischemiareperfusion notably increased oxidative stress, apoptosis, and inflammatory markers, as shown by higher levels of malondialdehyde, caspase-3, TNF-, and IL-6 ( p 0.05, and homogeneity of variance using Bartletts test before the ANOVA test

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To conclude, and accordingly to the data summarized here, melatonin is considered a potent molecule that has received increasing attention in the management of a large variety of diseases with an inflammatory etiology 7,70,71,72,73,79 (Fig