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Cells were suspended in HBSS, placed into black, clear-bottomed plates, and FRET was measured before and after agonist addition in a Flexstation 3 plate reader at 37C using the following settings: ex = 440 nm, em = 485 and 535 nm

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When used with insulin or sulfonylureas, dose reductions of these medications may be needed to reduce the risk of hypoglycaemia

"I now use it as I feel I need it, as a tool to manage not yo-yoing, she told People at the time

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It is the primary anabolic hormone in the body, facilitating glucose uptake into muscle, fat, and liver cells while inhibiting glucose production by the liver