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Piwowarczyk A, Horvath A, Pisula E, Kawa R, Szajewska H

After 24 h, the medium was discarded, and hepatocytes were cultured in a maintenance medium composed of seeding medium, supplemented by 50 M hydrocortisone hemisuccinate and 2% (vol/vol) DMSO, as previously described ( 41 )

IGF-1 LR3, also known as Long-Arg3-IGF-I, is a synthetic peptide similar to Insulin-like Growth Factor 1 (IGF-1)