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A 30 mg retatrutide vial with 3 mL of bacteriostatic water makes 10 mg/mL, where 8 mg draws 80 units and 10 mg draws 100 units

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Protein quantification was performed by densitometry analysis of whole iWAT or iWAT samples enriched in mitochondria separated on 12.5% SDSPAGE gels and using a low-molecular-weight protein calibration kit (Amersham, GE Healthcare)