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For Oil Red O Staining, liver tissues were frozen in Tissue-Tek CRYO-OCT (Fisher Scientific) and cryostat tissue sections were cut at 7 m thicknesses, fixed with 10% formalin for 10 min, and stained following an Oil Red O procedure

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By blocking the downstream pathways of key cytokines such as IFN, IL2, IL15, and IL17, it effectively suppresses T cell production and activation

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