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Try to stay calm and take deep breaths

The cell lysates were centrifuged at 12,000 g for 10 minutes and the supernatant was mixed with the FLAG antibody overnight at 4C on a shaker, before incubation with prewashed protein A/G agarose beads at 25C for 4 hours

whether treatment prior to insults would be relevant, route of administration, and concentration)

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