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Apoptosis of CNGB3MYC cDNA-transfected HEK293T cells was determined by TUNEL assay using the ApopTag immunoperoxidase kit (Chemicon, Temecula, CA)

Although, the induction of the Cdk1 mRNA expression was found relatively similar in both mice genotypes (Figure 5d), CDK1 protein, which is known to be expressed during DNA replication and progression in G2 and M phases, was transiently induced at 40 and 44 h in the WT livers while its expression increased progressively with maximal levels between 55 and 72 h in mutant mice (Figures 5b and c)

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The reagents were prepared as described in the kit

Erwin et al., 1990)