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After washing in PBS, horseradish peroxidase-conjugated goat anti-rabbit IgG (ab175470, Abcam) diluted 1:200 in 1% rabbit serum in PBS was applied, and the slides were incubated for 30 min at room temperature

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Authors contributions: Vignesh NR and Sakthi Megalai: Contributed to the definition of intellectual content and literature search, Balakumaran C and Sowbaghya PT: Contributed to data and statistical analysis as well as manuscript preparation

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Researchers alternating between both timings across different cycle phases report the strongest overall body recomposition, but that requires meticulous tracking and protocol adherence most people don't maintain

Human bodies produce ROS consisting of superoxide anion (O2-), hydrogen peroxide (H2O2), hydroxyl radicals (OH), singlet oxygen (1O2), lipid peroxide and nitrogen oxide (37)