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(B) Relationship between the melanosis score and the number of affected segments

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Irisin mediates effects on bone and fat via V integrin receptors

Samples were cleaned in wash solution (phosphate buffered saline (PBS) containing 5 Pen/Strep with antimycotic/antifungal) and hair was shaved off close to the epidermis

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In contrast, direct central mechanisms including PI3K/Akt-mediated neuroprotection, BDNF upregulation, and enhanced autophagy are primarily supported by preclinical data obtained at drug concentrations substantially higher than those achievable in the human brain at therapeutic doses