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The samples were then sonicated in ice-cold water using Biorupter UCD-200 sonicator for 5 min (30 s sonication and 30 s rest cycle

Characterizing steroid hormone receptor chromatin binding landscapes in male and female breast cancer

Two samples, AF488-ASPL alone (final concentration of 0.2 M) and AF488-ASPL (final concentration of 0.2 M) in a mixture with VCP (final concentration of 16.8 M), in 50 mM Hepes, 200 mM NaCl, 2 mM adenosine 5-triphosphate (ATP), 2 mM MgCl 2 , and 0.5 mM sodium vanadate buffer were loaded into cell assemblies with double-sector 12-mm charcoal-filled centerpieces and quartz windows

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