The resulting product from this reaction was measured using spectrophotometry (412 nm)
Healthy female mice served as pure controls
Microspheres were compared to Glutathione Agarose purchased from Gold Biotechnology
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It is a tripeptide, which means it is a small protein made of three specific amino acids: cysteine, glycine, and glutamic acid
Nevertheless, cystine is taken up by some cells (i.e., endothelial cells) and, given the high reducing conditions, is intracellularly reduced to cysteine, employed for the synthesis of GSH ( The last GSH synthetic process is the one depending on the conversion of the oxidized dimer GSSG to 2 reduced GSH molecules in cells by glutathione reductase (GR), an ubiquitous enzyme of the family of disulfide reductases, in the presence of NADPH and flavin adenine dinucleotide -FAD-
